The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR goods have been digested and cloned into a pET21a+ vector working with NdeI and XhoI restriction enzymes. A point mutation was introduced by the quick https://karlr988eoz1.activablog.com/26331461/xeruborbactam-for-dummies