The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned into a pET21a+ vector applying NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the fast alter https://foy-25191110.thelateblog.com/27018725/considerations-to-know-about-xeruborbactam